Journal: eLife

Article Title: High-phytate/low-calcium diet is a risk factor for crystal nephropathies, renal phosphate wasting, and bone loss

doi: 10.7554/elife.52709

Figure Lengend Snippet: Figure 6. Phytate-mediated Ca2+ deficiency promotes vitamin D insufficiency and renal phosphate wasting independent of FGF23 expression. (A–C) Time-course analysis of serum levels of intact PTH (A), 25(OH)D (B), and 1,25(OH)2D (C) in rats fed control, HP-LCa2+, and HP-HCa2+ diets. For the early measurement of 25(OH)D (B) and 1,25(OH)2D, we pooled the sera from 2 to 3 rats. (D–F) Time-course analysis of renal CYP27B1 (D), CYP24A1 (E), and VDR (F) in rats fed control, HP-LCa2+, and HP-HCa2+ diets. (G) Immunoblot analysis of renal aKlotho, NHERF1, NaPi-2a in rats fed control, HP-LCa2+, Figure 6 continued on next page

Article Snippet: Key resources table Reagent type (species) or resource Designation Source or reference Identifiers Additional information Genetic reagent (Rattus norvegicus) Sprague Dawley rat Orient Bio, Seoul, Korea Developed by Sprague Dawley, Inc. Chemical compound, drug AIN-93G Dyets Inc. DYET# 10700 Control diet Chemical compound, drug Phytic acid Sigma-Aldrich Cat. #: P-8810 Phytate diet Chemical compound, drug HNO3 Sigma-Aldrich Cat. #: 438073 Fecal mineral analysis Chemical compound, drug HF Sigma-Aldrich Cat. #: 695068 Fecal mineral analysis Chemical compound, drug HCl Sigma-Aldrich Cat. #: H1758 Fecal mineral analysis Chemical compound, drug EDTA Sigma-Aldrich Cat. #: 93283 Fecal phytate analysis Chemical compound, drug NaOH Sigma-Aldrich Cat. #: 415413 Fecal phytate analysis Chemical compound, drug Calcium Beckman Coulter OSR6113 Serum/urine biochemistry Chemical compound, drug Phosphate Beckman Coulter OSR6122 Serum/urine biochemistry Chemical compound, drug BUN (Urea) Beckman Coulter OSR6134 Serum/urine biochemistry Chemical compound, drug Creatinine Beckman Coulter OSR6178 Serum/urine biochemistry Chemical compound, drug Magnesium Beckman Coulter OSR6189 Serum/urine biochemistry Chemical compound, drug Urine protein Beckman Coulter OSR6170 Urine biochemistry Commercial assay or kit Rat intact PTH Immutopics Cat. #: 60–2500 ELISA kit Commercial assay or kit Human PTH Abcam Cat. #: ab230931 ELISA kit Commercial assay or kit Rat soluble RANKL Immundiagnostik Cat. #: K1019 ELISA kit Commercial assay or kit Rat osteoprotegerin Alpco Immunoassay Cat. #: 30–1020 ELISA kit Commercial assay or kit Rat intact FGF23 Elabscience Cat. #: E-EL-R3031 ELISA kit Commercial assay or kit Rat C-terminal FGF23 Elabscience Cat. #: E-EL-RB0377 ELISA kit Commercial assay or kit Human FGF23 Elabscience Cat. #: E-EL-H1116 ELISA kit Commercial assay or kit 25(OH)-Vitamin D Abbott/USA ARCHITECT 25-OH Vitamin D Chemiluminescence microparticle immunoassay Commercial assay or kit 1,25-(OH)two vitamin D DIAsoure 1,25(OH)2-VIT, D-RIA-CT/Belgium Radioimmunoassay Continued on next page Kim et al. eLife 2020;9:e52709.

Techniques: Expressing, Control, Western Blot

Journal: Regenerative Therapy

Article Title: Extracellular vesicles derived from adipose-derived mesenchymal stem/stromal cells prevent synovial inflammation and attenuate cartilage degeneration in rodent osteoarthritis

doi: 10.1016/j.reth.2025.101056

Figure Lengend Snippet: Differential gene expression besed on Single-cell RNA sequencing of joint cell clusters following extracellular vesicle (EVs) treatment . ( a-d ) Comparison of gene expression in EVs, PBS, and WT groups (a) in Cluster 6 (chondrocyte progenitors) (COL2A1, COL1A2, PRG4, MMP3, CCL2, and FGF18). ( b ) in Cluster 1 (NK cells) (CD14, MRC1, CD163, CD86, CD80, and NOS2). ( c ) in Cluster 8 (Mesenchymal cells) (COL2A1, COL1A2, PRG4, CCL2, and FGF18) ( d ) in Cluster 4 (Neutrophils) (APOE, AGPAT4, HAPLN1, CDKN1C, MET, and CEMIP2). (e) Flow cytometry analysis confirmed that the proportion of CD11b + CD163 + M2 macrophages was approximately 2.5-fold higher in the EVs-treated group compared to the PBS-treated group.

Article Snippet: After 24 h of stimulation, the supernatant was collected and the amount of Fibroblast Growth Factor 18 (FGF18) protein in the supernatant was measured using Human FGF18 ELISA set (Elabscience Biotechnology Inc.).

Techniques: Gene Expression, RNA Sequencing, Comparison, Flow Cytometry

Journal: Antibodies

Article Title: Two Highly Specific Mouse Monoclonal Antibodies to the Putative C-Telopeptide of Human Collagen XIα1, a Cancer Biomarker

doi: 10.3390/antib15020021

Figure Lengend Snippet: The amino acid sequence of the COL11A1 Fusion Protein (residues 1545 to 1806 of the P12107-1 A isoform), recombinantly expressed in Escherichia coli , after the removal of an N-terminal GST tag (provided by Proteintech). The first 19 N-terminal (1545) PLPILSSKKTRRHTEGMQA (1563) amino acid residues are part of the putative C-telopeptide. The next 243 amino acid residues (1564 to 1806) constitute the C-propeptide.

Article Snippet: The recombinant COL11A1 Fusion Protein from Proteintech was also assayed in an ELISA and Western blot with finally purified preparations of the anti-colXIα1 clone 9 and PLY-7 mAbs. shows their ELISA immunoreactivity characteristics.

Techniques: Sequencing

Journal: Antibodies

Article Title: Two Highly Specific Mouse Monoclonal Antibodies to the Putative C-Telopeptide of Human Collagen XIα1, a Cancer Biomarker

doi: 10.3390/antib15020021

Figure Lengend Snippet: SDS-PAGE gel staining and Western blot of recombinant antigens with finally purified preparations of anti-colXIα1 clone 9 and PLY-7 mAbs. Lane 1: PageRuler™ Plus Prestained Protein Ladder. Lane 2: COL11A1 Fusion Protein (Proteintech). Lane 3: Collagen XIα1 (GenScript). Lane 4: C-propeptide (GenScript). Western blot color development was monitored following the manufacturer’s instructions. Full-length blots/gels are presented in .

Article Snippet: The recombinant COL11A1 Fusion Protein from Proteintech was also assayed in an ELISA and Western blot with finally purified preparations of the anti-colXIα1 clone 9 and PLY-7 mAbs. shows their ELISA immunoreactivity characteristics.

Techniques: SDS Page, Staining, Western Blot, Recombinant, Purification

Journal: Antibodies

Article Title: Two Highly Specific Mouse Monoclonal Antibodies to the Putative C-Telopeptide of Human Collagen XIα1, a Cancer Biomarker

doi: 10.3390/antib15020021

Figure Lengend Snippet: The PEP-FOLD4-derived structural predictions of peptides related to the putative C-telopeptide. ( A ): The 50 N-terminal amino acid sequence of the COL11A1 Fusion Protein from Proteintech, with the first 19 N-terminal PLPILSSKKTRRHTEGMQA amino acid residues of the putative C-telopeptide. ( B ): A free RRHTEGMQA peptide. ( C ): The 50 C-terminal amino acid sequence of GenScript’s recombinant collagen XIα1 form, whose last 21 C-terminal IQPLPILSSKKTRRHTEGMQA amino acid residues correspond to the putative C-telopeptide. The peptide’s N-terminus is on the left in ( A , B ) and on the right in ( C ).

Article Snippet: The recombinant COL11A1 Fusion Protein from Proteintech was also assayed in an ELISA and Western blot with finally purified preparations of the anti-colXIα1 clone 9 and PLY-7 mAbs. shows their ELISA immunoreactivity characteristics.

Techniques: Derivative Assay, Sequencing, Recombinant

Journal: Avicenna Journal of Medical Biotechnology

Article Title: The Potential of Human Wharton’s Jelly Mesenchymal Stem Cells Secretome Based Topical Gel for Therapeutic Application

doi: 10.18502/ajmb.v16i4.16739

Figure Lengend Snippet: Effect of various treatments on hWJMSCs-Sec toward growth factor proteins level. A) KGF, B) HGF, C) HB-EGF, D) PDGF, E) EGF. The data were presented as mean ± standard deviation. Different superscript letters on Figure A (a, b, c, cd, d, e, f), and B, C, D, E (a, b, c, d, e, f, g) showed significant differences among treatments at p<0.05 (Data was analyzed using ANOVA followed by Tukey HSD post hoc test). Samples included hWJMSCs-Sec (S), Freeze-dried hWJMSCs-Sec (FDS), Freeze-dried Medium Basal (FDMB), Freeze-Dried hWJMSCs-Sec Gel (FDSG), and Freeze-dried Gel Based (FDGB). Number on samples indicate the ratio of gel to hWJMSCs-Sec, 1) 6 g : 3 ml , 2) 6 g : 4.5 ml , and 3) 6 g : 6 ml .

Article Snippet: The Human KGF (E-EL-H0092), HGF (E-EL-H0084), PDGF (E-EL-H2211), EGF (E-EL-H0059), and HB-EGF (E-EL-H2667) level were assayed using the Elabscience kit, based on manufacturing protocols.

Techniques: Standard Deviation

Journal: Avicenna Journal of Medical Biotechnology

Article Title: The Potential of Human Wharton’s Jelly Mesenchymal Stem Cells Secretome Based Topical Gel for Therapeutic Application

doi: 10.18502/ajmb.v16i4.16739

Figure Lengend Snippet: Proposed mechanism of hWJMSCs-Sec gel as a wound healing agent. * The treatment of hWJMSCs-Sec gel into the wounded skin can regulate some growth factors such as KGF, HGF, HB-EGF, PDGF, and EGF. The upregulation of these growth factors can induce the production of collagen, which plays a crucial role to regenerate tissue formation. The high antioxidant hWJMSCs-Sec gel also has the ability to scavenge free radicals, and inhibit the excessive inflammation that can cause irritation, redness, and pain in the wound.

Article Snippet: The Human KGF (E-EL-H0092), HGF (E-EL-H0084), PDGF (E-EL-H2211), EGF (E-EL-H0059), and HB-EGF (E-EL-H2667) level were assayed using the Elabscience kit, based on manufacturing protocols.

Techniques: